Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Solenoids01:17

Solenoids

3.2K
A solenoid is a conducting wire coated with an insulating material, wound tightly in the form of a helical coil. The magnetic field for a solenoid is the vector sum of the magnetic field due to its individual turns. For an ideal solenoid, the magnetic field inside is almost uniform and parallel to the solenoid axis, while the magnetic field outside the solenoid is nearly zero.
Each turn in a solenoid can be approximated as a circular current carrying coil that generates a dipole moment. The...
3.2K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.4K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.4K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.9K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.5K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.5K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.6K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.6K
Magnetic Field of a Solenoid01:18

Magnetic Field of a Solenoid

5.7K
A solenoid is a conducting wire coated with an insulating material, wound tightly in the form of a helical coil. The magnetic field due to a solenoid is the vector sum of the magnetic fields due to its individual turns. Therefore, for an ideal solenoid, the magnetic field within the solenoid is directly proportional to the number of turns per unit length and the current. Conversely, the magnetic field outside the solenoid is zero.
Consider a solenoid with 100 turns wrapped around a cylinder of...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Bothrops</i> venom variation drives niche-specific pharmacology through Ca<sup>2+</sup> signalling and membrane damage.

Frontiers in pharmacology·2026
Same author

Identification of DC-174 as a Novel Hydroxamic Precandidate for the Development of an Oral Snakebite Treatment.

Journal of medicinal chemistry·2026
Same author

Multiple Heart-Cut Ion-Exchange Chromatography-Reversed-Phase Liquid Chromatography Platform for Online Desalting and Fractionation of Monoclonal Antibody Charge Variants.

Journal of separation science·2026
Same author

Profiling cytotoxicity of nanofractionated elapid snake venoms in human cell lines representing different tissues.

Journal of pharmaceutical analysis·2026
Same author

Enabling Large-Volume Injections in Hydrophilic Interaction Chromatography of Oligonucleotides Through In-Line Mixing.

Journal of separation science·2026
Same author

Leveraging fragment-based drug discovery to advance 3D scaffolds into potent ligands: application to the histamine H<sub>1</sub> receptor.

RSC medicinal chemistry·2026
Same journal

Revolutionizing Transcriptomics: From Single-Cell Insights to RNA-based Interventions.

SLAS technology·2026
Same journal

Smartphone-based colorimetric glucose biosensor using peroxidase-like activity of bimetallic catalyst supported onto graphitic carbon nitride nanosheets.

SLAS technology·2026
Same journal

XVCF: Exquisite visualization of VCF data from genomic experiments.

SLAS technology·2026
Same journal

EasyPip: An equipment-agnostic software application to transform automated liquid handlers into efficient walk-up tools for routine plate-based pipetting.

SLAS technology·2026
Same journal

Identification of ubiquitination-related biomarkers in osteoarthritis: Combining transcriptome and Mendelian randomization analysis.

SLAS technology·2026
Same journal

Miniaturization of a Lumit p-ERK immunoassay for cell-based high-throughput screening of a chemogenetic small-molecule library.

SLAS technology·2026
See all related articles

Related Experiment Video

Updated: Jan 24, 2026

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
09:35

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Published on: July 17, 2018

9.4K

Compound Identification Using Liquid Chromatography and High-Resolution Noncontact Fraction Collection with a

Willem Jonker1, Koen de Vries1, Niels Althuisius2

  • 1Division of Bioanalytical Chemistry, Amsterdam Institute of Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, Amsterdam, Netherlands.

SLAS Technology
|May 18, 2019
PubMed
Summary
This summary is machine-generated.

A novel noncontact fraction collector, the FractioMate, enables high-resolution liquid chromatography (LC) fractionation for bioactivity screening and analyte isolation. This device ensures accurate, repeatable fraction collection for advanced analytical applications.

Keywords:
NMReffect-directed analysisfraction collectionliquid chromatographyreporter gene assaysolenoid valve

More Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.3K
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

12.3K

Related Experiment Videos

Last Updated: Jan 24, 2026

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
09:35

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Published on: July 17, 2018

9.4K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.3K
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

12.3K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Biotechnology

Background:

  • Traditional fraction collectors can lack precision for high-resolution separations.
  • Automated, high-density well plate fractionation is crucial for high-throughput screening.
  • Developing advanced tools for liquid chromatography (LC) is essential for modern bioanalysis.

Purpose of the Study:

  • To develop and characterize a high-resolution, noncontact fraction collector for LC.
  • To evaluate the performance of the FractioMate for accurate and repeatable fraction collection.
  • To demonstrate the utility of the FractioMate in bioactivity screening and analyte isolation.

Main Methods:

  • Development of the FractioMate using a low-dead-volume solenoid valve in a modified autosampler.
  • Systematic investigation of solenoid valve parameters (frequency, voltage) and LC conditions (flow rate, mobile phase).
  • Validation using mass spectrometry, reporter gene assays, and nuclear magnetic resonance (NMR) analysis.

Main Results:

  • The FractioMate achieved accurate, repeatable, and stable fraction collection across various LC conditions.
  • Demonstrated successful fractionation of complex samples (estrogenic/androgenic compounds, surface water) for bioactivity screening.
  • Showcased feasibility for isolating analytes for subsequent nuclear magnetic resonance (NMR) analysis.

Conclusions:

  • The FractioMate is a versatile and high-performance fraction collector for demanding LC applications.
  • The noncontact, high-resolution fractionation capability enhances bioactivity screening and preparative LC.
  • This technology advances automated sample handling in analytical and biochemical research.