Related Experiment Video

Updated: Jan 24, 2026

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.4K

Inhibition of SMYD2 Sensitized Cisplatin to Resistant Cells in NSCLC Through Activating p53 Pathway

Lei Shang1,2, Minjie Wei1

  • 1School of Pharmacy, China Medical University, Shenyang, China.

Frontiers in Oncology
|May 21, 2019
PubMed

Insights

SMYD2 inhibition enhances cisplatin sensitivity in non-small cell lung cancer (NSCLC) by activating the p53 pathway. This finding suggests SMYD2 as a potential therapeutic target for overcoming cisplatin resistance in NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • SMYD2 (the 'another proline-rich domain protein' methyltransferase) is implicated in gene transcription, signaling, and tumor progression.
  • The role of SMYD2 in acquired drug resistance, particularly in non-small cell lung cancer (NSCLC), remains largely unexplored.

Purpose of the Study:

  • To investigate the role of SMYD2 in cisplatin resistance in non-small cell lung cancer (NSCLC).
  • To explore the potential of targeting SMYD2 as a therapeutic strategy to overcome cisplatin resistance in NSCLC.

Main Methods:

  • Utilized specific SMYD2 inhibitors and siRNA for knockdown experiments in NSCLC and cisplatin-resistant NSCLC cell lines (NSCLC/CDDP).
  • Assessed cell sensitivity to various chemotherapeutic agents, including cisplatin (CDDP).
  • Investigated effects on tumor sphere formation, cell migration, apoptosis, and the p53 signaling pathway.

Main Results:

  • SMYD2 inhibition sensitized NSCLC cells to cisplatin but not other tested drugs.
  • SMYD2 and its substrates were overexpressed in resistant NSCLC cells; inhibition/knockdown reversed resistance.
  • SMYD2 targeting inhibited tumor sphere formation and migration in resistant cells, enhanced p53 pathway activity, and induced apoptosis.
  • p53 pathway modulation directly impacted cisplatin sensitivity, confirmed by in vivo studies.

Conclusions:

  • SMYD2 plays a critical role in conferring cisplatin resistance in NSCLC through regulation of the p53 pathway.
  • Targeting SMYD2 presents a promising therapeutic avenue for overcoming cisplatin resistance in non-small cell lung cancer.
Keywords:
SMYD2apoptosiscisplatin resistancelung cancerp53

More Related Videos

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.6K
Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.8K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.0K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
48.9K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.4K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
5.7K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
91.8K