Physical and chemical stability of β-carotene nanoemulsions during storage and thermal process

Nanotechnology has become an option for the encapsulation of compounds, such as carotenoids. However, for the incorporation in to food, it is necessary to develop nanometric systems that are stable under the different conditions to which the food is submitted during its production, transport, and storage. Thus, with the intent to develop a stable nanoemulsion formulation for food application, the physical and chemical stability of β-carotene nanoemulsions after thermal treatments and storage under different conditions, were investigated in this work. The β-carotene nanoemulsions were formulated with corn oil, by applying high-pressure homogenization, with an average size in the 300 nm range, which is within the appropriate scale for industrial preparations, such as foods and cosmetics. The nanoemulsion droplets had negative charge (more than -25 mV) and monodisperse profile. The sample were pasteurized, sterilized, and stored at 4, 25, and 37 °C in the presence and absence of light for up to 90 days. Following the heat treatments and storage, the nanoemulsions showed no evidence of physical destabilization, retaining 70-80% of the carotenoid after the pasteurization and sterilization processes, and 70% when stored at 4 °C without light, respectively. Overall, our findings provide new information about the physical and chemical stability of β-carotene nanoemulsions during traditional thermal processes and environmental conditions.