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Updated: Jan 24, 2026
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Published on: October 31, 2016
8.5K
Cobalt-chromium-enriched medium ameliorates shear-stressed endothelial cell performance
Mariana Issler Pinheiro Machado1, Anderson Moreira Gomes1, Marcel Ferreira Rodrigues1
1Department of Chemistry and Biochemistry, Bioscience Institute, Sao Paulo State University, UNESP, Campus Botucatu, Botucatu, São Paulo, Brazil.
Summary
Cobalt-chromium (CoCr) released molecules influence endothelial cells, impacting extracellular matrix remodeling and intracellular signaling pathways. This suggests a role for CoCr in angiogenesis, crucial for bone healing with implantable devices.
Area of Science:
- Biomaterials Science
- Cell Biology
- Tissue Engineering
Background:
- Angiogenesis is vital for bone healing and the success of implantable devices.
- Cobalt-chromium (CoCr) alloys are promising for biomedical applications.
- Implant-released molecules may affect endothelial cells, influencing healing.
Purpose of the Study:
- To investigate the effects of CoCr-enriched medium on endothelial cells (HUVECs).
- To explore intracellular pathways involved in cytoskeletal rearrangement, cell viability, and ECM remodeling under shear stress.
- To understand the role of CoCr in angiogenesis-related mechanisms.
Main Methods:
- Culturing HUVECs in CoCr-enriched medium under shear stress.
- Analyzing metalloproteinases (MMPs) activity.
- Assessing mRNA and protein expression of MAPK family members, CDK4, P15, and P21.
Main Results:
- CoCr-enriched medium induced significant extracellular matrix remodeling.
- CoCr modulated mRNA and protein expression of MAPK family members.
- Expression of CDK4, P15, and P21 genes was altered, indicating regulatory mechanisms.
Conclusions:
- Trace amounts of CoCr elements trigger intracellular signaling in shear-stressed endothelial cells.
- CoCr influences angiogenesis-related mechanisms, particularly ECM remodeling.
- These findings offer insights into the biological activity of CoCr in biomedical applications.
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