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MicroRNA-628-5p inhibits cell proliferation in glioma by targeting DDX59

Peng Xie1, Yan Wang2, Yuanmei Liao3

  • 1Department of Neurosurgery, The Second People's Hospital of Huai'an, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.

Journal of Cellular Biochemistry
|May 22, 2019
PubMed

Insights

MicroRNA-628-5p (miR-628-5p) is downregulated in glioma, inhibiting cancer cell proliferation and survival. Restoring miR-628-5p levels may offer a novel therapeutic strategy for glioma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • microRNA-628-5p (miR-628-5p) has been implicated in epithelial ovarian cancer.
  • The specific role and mechanisms of miR-628-5p in glioma remain largely unexplored.

Purpose of the Study:

  • To investigate the biological functions and underlying mechanisms of miR-628-5p in glioma development.
  • To determine if miR-628-5p could serve as a potential therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR to assess miR-628-5p expression in glioma tissues and cell lines (U87, T98).
  • Cell proliferation assays and cell cycle analysis to evaluate the functional impact of miR-628-5p.
  • Western blotting to measure protein levels of DDX59 and p-AKT.
  • Luciferase reporter assays to confirm direct binding of miR-628-5p to the DDX59 3'-UTR.

Main Results:

  • miR-628-5p expression was significantly decreased in glioma tissues and cells compared to normal controls.
  • Overexpression of miR-628-5p suppressed glioma cell proliferation and induced G1 cell cycle arrest.
  • miR-628-5p directly targets DDX59, leading to reduced DDX59 protein levels and subsequently decreased p-AKT.
  • Restoration of DDX59 expression attenuated the inhibitory effects of miR-628-5p on glioma cell proliferation.

Conclusions:

  • The miR-628-5p/DDX59 axis plays a critical role in glioma pathogenesis.
  • miR-628-5p functions as a tumor suppressor in glioma by inhibiting proliferation and promoting cell cycle arrest.
  • miR-628-5p represents a promising novel therapeutic target for glioma treatment.
Keywords:
DDX59cell cycle arrestgliomamiR-628-5pproliferation

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