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Area of Science:

  • Food safety analysis
  • Analytical chemistry
  • Mass spectrometry

Background:

  • Regulated, modified, and emerging mycotoxins frequently co-occur in food and feed globally.
  • Accurate and validated methods for simultaneous multimycotoxin determination are essential for regulatory compliance and surveillance.
  • Liquid chromatography-mass spectrometry (LC-MS) is increasingly used for multimycotoxin screening and quantification in control laboratories.

Purpose of the Study:

  • To provide insights into guidelines and tools for evaluating the performance of LC-MS methods for multimycotoxin determination.
  • To critically review performance criteria for LC-MS methods established in the EU and US.
  • To discuss future challenges and needs in LC-MS based multimycotoxin analysis.

Main Methods:

  • Review of existing LC-MS methodologies for multimycotoxin screening and quantification.
  • Analysis of European Standards and regulatory criteria for mycotoxin detection methods.
  • Critical overview of performance evaluation guidelines and tools for LC-MS methods.

Main Results:

  • Currently, only one European Standard exists for LC-MS based multimycotoxin determination (EN 16877:2016).
  • Several LC-MS methods for multimycotoxin determination are under approval as European Standards.
  • The European Commission has defined specific criteria for regulatory use of mycotoxin screening methods, including LC-MS.

Conclusions:

  • Standardized LC-MS methods are urgently needed for reliable multimycotoxin determination, as highlighted by regulatory bodies.
  • Performance evaluation of LC-MS methods is critical for ensuring accuracy and reliability in food and feed safety.
  • Addressing future challenges will enhance the capabilities of LC-MS for comprehensive mycotoxin surveillance.