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Chrysin inhibits sphere formation in SMMC-7721 cells via modulation of SHP-1/STAT3 signaling pathway

Yanqin Zhang1,2, Feng Chen1, Xinghua Xiao1

  • 1Department of Pharmacy, Xiangya Hospital of Central South University, Changsha, 410008, People's Republic of China.

Cancer Management and Research
|May 23, 2019
PubMed

Insights

Chrysin, a natural flavonoid, inhibits hepatocellular carcinoma (HCC) sphere formation by upregulating SHP-1 protein and modulating the SHP-1/STAT3 pathway, offering potential anti-cancer effects.

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Chrysin, a natural flavonoid, exhibits anti-cancer properties against various cancer cells.
  • Chrysin has shown potential in inhibiting sphere formation in hepatocellular carcinoma (HCC) cell lines.
  • The precise mechanism of chrysin's action on HCC spheres is not fully understood.

Purpose of the Study:

  • To investigate the effect of chrysin on SHP-1 and STAT3 signaling in HCC.
  • To elucidate the mechanism by which chrysin inhibits HCC sphere formation.

Main Methods:

  • Investigated SHP-1 protein expression in HCC spheres.
  • Assessed the impact of chrysin on SHP-1, p-STAT3, and Twist1 expression.
  • Utilized SHP-1 knockdown to validate its role in chrysin's anti-sphere formation effects.

Main Results:

  • SHP-1 protein expression was decreased in HCC spheres.
  • Chrysin treatment significantly inhibited sphere formation and increased SHP-1 levels.
  • Chrysin reduced p-STAT3 and Twist1 expression; SHP-1 knockdown reversed these effects and chrysin's inhibition of sphere formation.

Conclusions:

  • Chrysin effectively inhibits HCC sphere formation.
  • The mechanism involves the modulation of the SHP-1/STAT3 signaling pathway.
  • Chrysin shows promise as a therapeutic candidate for HCC treatment.
Keywords:
SHP-1STAT3chrysinhepatocellular carcinomasphere formation

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