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Updated: Jan 24, 2026

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy
Published on: April 29, 2014
MiR-376a functions as tumor suppressor by targeting SGK3 in renal cell carcinoma
1Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Dongcheng District, Beijing, P. R. China. quanzong_mao@163.com.
Objective:
Emerging evidence showed that microRNA-376a (miR-376a) functions as a crucial role in human cancers. However, its role in renal cell carcinoma (RCC) remains unclear.
Materials And Methods:
MiR-376a expression in RCC cell lines was analyzed by quantitative real time-polymerase chain reaction (qRT-PCR). The target of miR-376a was validated using the luciferase activity reporter assay, and the effects of miR-376a expression on RCC cell behaviors were investigated in vitro.
Results:
MiR-376a expression was downregulated in RCC cell lines in comparison with HK-2 cell line. Low miR-376a expression was correlated with poor overall survival of RCC patients. Serum/glucocorticoid regulated kinase family member 3 (SGK3) was validated as a direct target of miR-376a. The overexpression of miR-376a inhibits RCC cell proliferation, migration, and invasion through regulating SGK3.
Conclusions:
Taken together, these results demonstrated the tumor suppressive role of miR-376a via targeting SGK3 in RCC and indicated that miR-376a might represent a novel therapeutic target for RCC treatment.
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