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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
Published on: January 20, 2022
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Catanionic Surfactant Vesicles as a New Platform for probing Glycan-Protein Interactions
Amanda Mahle1, Neeraja Dashaputre2, Philip DeShong2
1Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742.
Advanced Functional Materials
|May 24, 2019
Summary
Researchers developed novel glycan-functionalized catanionic vesicles (CVs) for carbohydrate synthesis and studying protein-glycan interactions. This platform enables high-throughput glycan array creation for biological research.
Area of Science:
- Carbohydrate Chemistry
- Biotechnology
- Glycobiology
Background:
- Glycomics research is hindered by challenges in synthesizing and analyzing complex carbohydrates.
- A scalable system for expressing known carbohydrate structures is needed for studying molecular interactions.
Purpose of the Study:
- To characterize glycan-functionalized catanionic vesicles (CVs) as a platform for glycan synthesis.
- To demonstrate the utility of these CVs as a scaffold for investigating protein-glycan interactions.
Main Methods:
- Utilized N. gonorrhoeae lipooligosaccharide (LOS) glycosyltransferase LgtE to modify LOS and glucose-functionalized CVs.
- Characterized CVs using differential lectin binding via flow cytometry.
- Assessed LgtE activity on whole cells and functionalized vesicles.
- Demonstrated ink-jet printability of CVs.
Main Results:
- LgtE exhibited similar biochemical properties when acting on whole cells versus LOS-functionalized vesicles.
- Glycan-functionalized CVs were successfully created and characterized.
- The platform demonstrated potential for high-specificity and high-throughput analysis.
Conclusions:
- Glycan-functionalized catanionic vesicles offer a promising platform for carbohydrate synthesis.
- This approach facilitates the development of glycan arrays for studying protein-glycan interactions.
- The characterized CVs represent a significant advancement for glycomics research.
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