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Updated: Jan 24, 2026

4D Imaging of Protein Aggregation in Live Cells
Published on: April 5, 2013
Unusual fluorescence of o-phenylazonaphthol derivatives with aggregation-induced emission and their use in two-photon
Yeoju Yoon1, Seonyoung Jo, Sang Jun Park
1Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 34134, Korea. tslee@cnu.ac.kr.
Abstract:
o-Phenylazonaphthol (o-PAN) derivatives including 6-bromo-1-((4-bromophenyl)diazenyl)naphthalen-2-ol (AN-Br-OH) and 1-phenylazo-2-naphthol (AN-OH, known as Sudan I (Color Index 12055)) were synthesized to investigate their fluorogenic behaviors, in which their aggregated-induced emission (AIE) is reported. The o-PANs showed a two-photon absorption. The protection of hydroxyl groups in o-PANs was used for fluorescence imaging of esterase-expressed HepG2 cells, which is potentially suitable for sensing and two-photon cell imaging applications.
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