Related Experiment Video
Updated: Jan 24, 2026
07:26
Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
Published on: March 4, 2020
6.0K
Transformation in Neisseria gonorrhoeae
Melanie M Callaghan1, Joseph P Dillard2
1Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2019
Summary
This chapter details laboratory methods for transforming Neisseria bacteria, including pathogenic and nonpathogenic species. It covers transformation mechanisms, DNA design, experimental procedures, and identifying successful transformants.
Area of Science:
- Microbiology
- Molecular Biology
- Bacterial Genetics
Background:
- Pathogenic Neisseria species exhibit well-established laboratory transformation techniques.
- These transformation methods have recently been extended to certain nonpathogenic Neisseria species.
Purpose of the Study:
- To provide foundational knowledge on Neisseria transformation.
- To guide researchers in DNA transformation substrate design.
- To present laboratory protocols for Neisseria transformation and transformant identification.
Main Methods:
- Exploration of the natural transformability mechanism in Neisseria.
- Design considerations for DNA transformation substrates.
- Detailed protocols for two distinct Neisseria laboratory transformation methods.
- Guidelines for confirming successful transformants.
Main Results:
- Established laboratory techniques for transforming pathogenic Neisseria.
- Successful application of transformation techniques to nonpathogenic Neisseria species.
- Comprehensive information on transformation mechanisms and substrate design.
Conclusions:
- Laboratory transformation is a viable technique for both pathogenic and nonpathogenic Neisseria.
- Understanding transformation mechanisms is key to successful experimental design.
- Standardized methods facilitate the genetic manipulation of Neisseria species.
More Related Videos
08:04Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Published on: February 8, 2019
9.1K
10:12Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
Published on: July 14, 2020
2.7K
Related Concept Videos
01:33Bacterial Transformation
59.5K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.5K
01:26Transformation
764
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
764
01:26Transformers
1.8K
A device that transforms voltages from one value to another using induction is called a transformer. A transformer consists of two separate coils, or windings, wrapped around the same soft iron core. However, they are electrically insulated from each other.
The iron core has a substantial relative permeability. Therefore, the magnetic field lines generated due to the current in one winding are almost entirely confined within the core, such that the same magnetic flux permeates each turn of both...
The iron core has a substantial relative permeability. Therefore, the magnetic field lines generated due to the current in one winding are almost entirely confined within the core, such that the same magnetic flux permeates each turn of both...
1.8K
01:26The Ideal Transformer
1.4K
In single-phase two-winding transformers, two windings are coiled around a magnetic core characterized by cross-sectional area A and magnetic permeability μ. A phasor current i1 enters the left winding while i2 exits the right winding, establishing the fundamental working of the transformer through electromagnetic principles.
Ampere's Law forms the basis of understanding the magnetic field within the transformer. It states that the integral of the magnetic field intensity's tangential...
Ampere's Law forms the basis of understanding the magnetic field within the transformer. It states that the integral of the magnetic field intensity's tangential...
1.4K
01:16Properties of the z-Transform II
401
The property of Accumulation in signal processing is derived by analyzing the accumulated sum of a discrete-time signal and using the time-shifting property to determine its z-transform. This principle reveals that the z-transform of the summed signal is related to the z-transform of the original signal by a multiplicative factor.
Moreover, the convolution property indicates that the convolution of two signals in the time domain corresponds to the product of their z-transforms in the frequency...
Moreover, the convolution property indicates that the convolution of two signals in the time domain corresponds to the product of their z-transforms in the frequency...
401
01:19Three-Winding Transformers
698
Three identical single-phase transformers can be configured to form a three-phase transformer connection, which involves high-voltage and low-voltage windings. The high-voltage windings are denoted by capital letters A-B-C, while the low-voltage windings are labeled with lowercase letters a-b-c, representing their respective phases. This notation helps distinguish between the high and low voltage sides of the transformer.
In the per-unit equivalent circuit of a grounded Y-Y three-phase...
In the per-unit equivalent circuit of a grounded Y-Y three-phase...
698