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Updated: Jan 24, 2026

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
Published on: March 4, 2020
Experimental Human Infection with Neisseria gonorrhoeae
Marcia M Hobbs1, Joseph A Duncan2
1Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. marcia_hobbs@med.unc.edu.
Abstract:
Experimental infection of male volunteers with Neisseria gonorrhoeae is safe and reproduces the clinical features of naturally acquired gonococcal urethritis. The human model is useful for testing the importance of putative gonococcal virulence factors for urethral infection in men and the model presents opportunities to examine host immune responses that may be exploited or improved in development and testing of gonococcal vaccines. In this chapter, we describe methods for production, characterization, and storage of N. gonorrhoeae stocks for experimental human challenge, preparation and delivery of inoculum suspensions, monitoring experimental infection, and statistical considerations for data analysis.
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