Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Affect in Interpersonal Attraction01:24

Role of Affect in Interpersonal Attraction

220
Affect plays a crucial role in shaping interpersonal evaluations and perceptions. Emotions influence how individuals judge and respond to others, often determining whether interactions are viewed positively or negatively. This effect can manifest directly through interactions with the person in question or indirectly via associations with unrelated emotional experiences.Direct Effects of Affect on AttractionAffect directly influences interpersonal attraction when a person’s behavior...
220
The Influence of Affect on Cognition01:29

The Influence of Affect on Cognition

273
Positive affect significantly influences cognitive processes, including evaluation, memory, creativity, and social judgments. Compared to negative affect, positive emotional states promote more favorable interpretations of stimuli, cognitive flexibility, and heuristic processing. These effects highlight emotions' powerful role in shaping how individuals perceive, remember, and interact with the world.Influence on Evaluation and AttributionWhen individuals experience positive affect, they are...
273
The Influence of Cognition on Affect01:29

The Influence of Cognition on Affect

196
Cognition plays a pivotal role in shaping emotional experiences, as demonstrated by Schachter and Singer’s two-factor theory of emotion. According to this model, emotion arises from a combination of physiological arousal and cognitive interpretation. The body’s physiological response to stimuli is ambiguous and only gains emotional significance through cognitive labeling. For instance, an increased heart rate and adrenaline surge while standing near an attractive person may be...
196
Background and Environment Affect Phenotype02:27

Background and Environment Affect Phenotype

7.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.5K
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

1.6K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.6K
Cellular Differentiation00:57

Cellular Differentiation

5.2K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cosurfactant-Induced Disorder in Polymersome Membrane Enhances Diffusion of Cargo Molecules.

ACS nano·2026
Same author

Printable Conductive Hydrogels for Electrochemical Biosensing and Soft Bioelectronic Interfaces.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Incorporation of Novel Synthetic Glycolipids in Liposomal Nanoparticles Affects Opsonization and In Vivo Clearance.

Angewandte Chemie (International ed. in English)·2026
Same author

Peptide-based artificial cytoskeleton enhances colocalized cascade reactions in cell-like microreactors.

Chemical science·2026
Same author

Macrophage Phenotype-Dependent Protein Corona Formation Governs Ligand Accessibility and Immune Clearance of Biomimetic Nanoparticles.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Sequence-Modulated Active Tripeptide Condensates for Tandem Catalysis.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Jan 24, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

9.2K

Protein deglycosylation can drastically affect the cellular uptake.

Artur Ghazaryan1, Katharina Landfester, Volker Mailänder

  • 1Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. katharina.landfester@mpip-mainz.mpg.de.

Nanoscale
|May 24, 2019
PubMed
Summary

The study reveals that sugars on nanocarrier protein coatings significantly alter cellular uptake. Removing sugars from clusterin and apolipoprotein AI changed how nanocarriers interact with cells, impacting targeted drug delivery.

More Related Videos

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.3K
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

5.3K

Related Experiment Videos

Last Updated: Jan 24, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

9.2K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.3K
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

5.3K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Nanocarriers are crucial for targeted drug delivery.
  • Upon entering biological fluids, nanocarriers form a biomolecular corona, influencing cellular interactions.
  • The protein corona's role is well-studied, but non-protein components like sugars are less understood.

Purpose of the Study:

  • To investigate the impact of protein glycosylation (sugars) on the biomolecular corona.
  • To determine how glycosylation affects nanocarrier cellular uptake.

Main Methods:

  • Nanocarriers were incubated in biological fluids to form a biomolecular corona.
  • Specific proteins, clusterin (CLU) and apolipoprotein AI (Apo AI), were isolated.
  • Glycosylation of these proteins was altered (deglycosylation).
  • Cellular uptake of nanocarriers with modified protein coronas was quantified.

Main Results:

  • Deglycosylation of clusterin (CLU) significantly increased nanocarrier cellular uptake.
  • Deglycosylation of apolipoprotein AI (Apo AI) significantly decreased nanocarrier cellular uptake.
  • Protein glycosylation plays a critical role in modulating nanocarrier-cell interactions.

Conclusions:

  • The study highlights the importance of non-protein components, specifically sugars, in the biomolecular corona.
  • Understanding protein glycosylation is essential for optimizing nanocarrier design for targeted drug delivery.
  • Modulating glycosylation offers a potential strategy to control nanocarrier biodistribution and efficacy.