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Changing microRNA Expression during Three-Month Wasp Venom Immunotherapy
Krzysztof Specjalski1, Agnieszka Maciejewska2, Ryszard Pawłowski2
1Department of Allergology, Medical University of Gdansk , Gdansk , Poland.
Immunological Investigations
|May 25, 2019
Summary
MicroRNAs regulate allergic diseases. Wasp venom immunotherapy (VIT) altered microRNA expression, suggesting a role in developing tolerance to wasp venom allergy.
Area of Science:
- Immunology
- Molecular Biology
Background:
- MicroRNAs are key regulators in allergic diseases.
- Their function in tolerance induction is not well understood.
Purpose of the Study:
- To investigate microRNA expression changes during wasp venom immunotherapy (VIT).
Main Methods:
- Analyzed microRNA expression in 5 allergic patients and 5 healthy controls.
- Collected blood samples before, during, and after VIT.
- Utilized microfluidic cards for expression profiling of 96 microRNAs.
Main Results:
- Wasp venom allergic patients showed higher miR-601 and miR-1201 expression compared to controls.
- VIT led to decreased expression of 6 microRNAs and increased expression of 11 microRNAs.
- Specific microRNAs like miR-182, miR-342, miR-375, let-7d, miR-34b, and miR-143 showed altered expression.
Conclusions:
- Wasp venom immunotherapy induces changes in microRNA expression profiles.
- These alterations are linked to Th2-type inflammation and the development of immune tolerance.
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