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Updated: Jan 24, 2026

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
Published on: March 14, 2014
Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability
Alexa Lindauer1, Tiffany May1, Gabriela Rios-Sotelo1
1Department of Biology, University of Nevada, Reno, 1664 North Virginia Street, Reno, NV, 89557, USA.
Abstract:
The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens' growth and reproduction to improve our understanding of chytridiomycosis.
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