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Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation
Valeria Kizner1, Sandra Fischer2, Benjamin Jähnke2
1CNS Diseases Research, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany. valeria.kizner@boehringer-ingelheim.com.
Abstract:
FLIPR-based calcium assay enables the detection and characterization of neuronal excitability by using electrical field stimulation to evoke and record action potential-driven calcium transients in induced pluripotent stem cell (iPSC)-derived cortical forebrain neurons. Here we describe high throughput measurement of neuronal excitability with a defined electrostimulation paradigm in a 384-well plate format using FLIPR.
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