Related Experiment Video
Updated: Jan 24, 2026

DNA Stable-Isotope Probing DNA-SIP
Published on: August 2, 2010
Highly sensitive aflatoxin B1 sensor based on DNA-guided assembly of fluorescent probe and TdT-assisted DNA
Bin Wang1, Jiushang Zheng1, Ailing Ding1
1Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, School of Materials and Energy, Southwest University, Chongqing 400715, PR China; Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Abstract:
A novel aptasensor, based on a perylene probe (PAPDI; N,N'-bis(propylenetrimethylammonium)-3,4,9,10-perylenediimide), was developed for the detection of aflatoxin B1 (AFB1) in maize samples. AuNPs/DNA composites were synthesized and integrated with aptamers-modified magnetic nanoparticles (MNPs) via DNA hybridization. For AFB1 determination, AuNPs/DNA composites were released from MNPs through specific binding of AFB1 with the aptamer and used for assembly of the PAPDI probe. To enhance the method sensitivity, terminal deoxynucleotidyl transferase (TdT)-catalyzed DNA polymerization was performed to elongate DNA on AuNPs/DNA composites. As a result, more PAPDI probes were assembled on the AuNPs/DNA composites. Through a multiple signal amplification strategy, the proposed method exhibited high sensitivity towards AFB1, with a detection limit of 0.01 nM (3.1 pg/mL). In summary, the proposed method has great potential to be a universal strategy for monitoring AFB1 in food samples.
Related Concept Videos
DNA Base Pairing
DNA Base Pairing
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
From DNA to Protein
DNA Replication
Replication in Prokaryotes
DNA replication...
Overview of DNA Repair
Chemically...

