Related Experiment Video

Updated: Jan 24, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

6.6K

Analyses of Cholesterol and Derivatives in Ocular Tissues Using LC-MS/MS Methods

Maria Piqueras1, Despoina Theotoka1, Gurkaran S Sarohia2

  • 1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2019
PubMed

Keywords:
CholesterolCholesterol estersElectrospray ionizationLipidomicsMass spectrometryOrbitrap™

More Related Videos

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.6K
Induction of Ocular Surface Inflammation and Collection of Involved Tissues06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

2.8K

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
1.3K
Measurement: Derived Units03:02

Measurement: Derived Units

The International System of Units or SI system, by international agreement, has fixed measurement units for seven fundamental properties: length, mass, time, temperature, electric current, amount of substance, and luminosity. These are called the SI base units.
54.6K
Tissues01:18

Tissues

Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
84.9K
The Scientific Method03:50

The Scientific Method

Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion. 
65.4K
First Derivative Test: Problem Solving01:25

First Derivative Test: Problem Solving

Imagine an asset price that crashes to a low point, rebounds sharply as bargain-hunters step in, and then gradually declines. Such behavior can be modeled with a smooth function whose turning points represent locally overvalued and undervalued regions. A convenient example that captures rebound followed by decay is:The high and low points of this curve are identified using the first derivative test, which determines where the function changes from increasing to decreasing or vice versa. To...
59
Second Derivative Test: Problem Solving01:24

Second Derivative Test: Problem Solving

In mathematical analysis, finding a function's highest and lowest points is crucial for understanding its behavior. These points, known as critical points, occur where the first derivative is either zero or undefined. Critical points are potential local maxima and minima locations, which can be classified using the Second Derivative Test. However, not every critical point corresponds to a local maximum or minimum. The second derivative is analyzed to classify these points. The second derivative...
85