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[Effect of pesticides on bacterial membranes]
Abstract:
The effect of pure preparation of ordram, fosalon, DDT, methoxychlorine, hydrel, dihydrel, 2,4-D, 2M-4C and of technical preparations of saturn, linuron, ronstar and keltan on the membrane functions (respiration and motility) of Azospirillum brasilense and Chromatium minutissimum cells and on malate and NADH oxidation by the isolated membranes of Micrococcus lysodeikticus was investigated. The effect varied from irreversible impairment to undetectable impairment of the measured activities depending on the type of bacteria and on the chemical used. The ordram induced permeability of the A. brasilense membranes without inhibition of the respiratory chain activity and selective inhibition of malate oxidase accompanied by stimulation of NADH oxidation in the M. lysodeikticus membranes indicates a possibility of ordram application as a regulator of bacterial metabolism.
Insights
This study investigated the impact of various pesticides on bacterial membrane functions. Ordram showed potential as a bacterial metabolism regulator by altering membrane permeability and enzyme activity.
Area of Science:
- Environmental Microbiology
- Biochemistry
Context:
- Pesticides can impact non-target organisms, including bacteria.
- Understanding these effects is crucial for environmental risk assessment.
Purpose:
- To investigate the effects of specific pesticides on bacterial membrane functions.
- To assess the impact on respiration, motility, and enzyme oxidation in different bacterial species.
Summary:
- Various pesticides (ordram, fosalon, DDT, methoxychlorine, hydrel, dihydrel, 2,4-D, 2M-4C, saturn, linuron, ronstar, keltan) were tested.
- Effects on Azospirillum brasilense, Chromatium minutissimum, and Micrococcus lysodeikticus varied from irreversible damage to no detectable impact.
- Ordram uniquely induced permeability in A. brasilense without inhibiting respiration and selectively affected M. lysodeikticus membrane enzymes.
Impact:
- Pesticide effects on bacterial membrane functions are chemical- and species-specific.
- Ordram's specific actions suggest potential applications in regulating bacterial metabolism.