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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Depth Perception and Spatial Vision01:15

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Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.
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Chromatographic Resolution01:15

Chromatographic Resolution

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In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
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In chromatography,...
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Visual System01:26

Visual System

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Light enters the eye through the cornea, a transparent, dome-shaped surface covering the surface of the eyeball that helps to direct and focus incoming light. This light is then channeled toward the pupil, an adjustable opening whose size is controlled by the iris. The iris, a pigmented muscle, regulates the amount of light entering the eye by contracting or dilating the pupil, thereby ensuring optimal light levels for clear vision.
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Racemic Mixtures and the Resolution of Enantiomers02:30

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A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit...
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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Updated: Jan 24, 2026

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy
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A guide to visualizing the spatial epigenome with super-resolution microscopy.

Jianquan Xu1, Yang Liu1

  • 1Biomedical Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

The FEBS Journal
|May 26, 2019
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy now visualizes higher-order chromatin structure in single cells. This advances spatial epigenomics, revealing genome function in health and disease.

Keywords:
DNA methylationchromatin organizationepigenetichistone modificationsuper-resolution

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Area of Science:

  • Cell Biology
  • Genetics
  • Microscopy

Background:

  • Eukaryotic genomic DNA is compacted into chromatin, a dynamic structure regulated by epigenetic modifications.
  • Conventional methods infer chromatin structure indirectly, lacking spatial detail of epigenomics.
  • Understanding chromatin's higher-order structure is crucial for development, aging, and cancer research.

Purpose of the Study:

  • To review the application of super-resolution microscopy for visualizing higher-order chromatin structure.
  • To explore the potential of spatial epigenomics for understanding genome function.
  • To highlight the integration of advanced microscopy and genomics.

Main Methods:

  • Super-resolution fluorescence microscopy for in vivo visualization of chromatin structure.
  • Analysis of epigenomic states within single cells.
  • Integration with high-throughput genomic technologies.

Main Results:

  • Super-resolution microscopy enables unprecedented resolution of in vivo higher-order chromatin structure.
  • Direct visualization of spatial epigenomics in single cells is now possible.
  • Preserves spatial context within the tissue microenvironment.

Conclusions:

  • Super-resolution microscopy offers new opportunities to study 3D chromatin structure and spatial epigenomics.
  • Synergistic integration with high-throughput genomics will advance understanding of genome function.
  • This approach is vital for studying normal biology and diseases like cancer and aging.