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Updated: Jan 24, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
Published on: March 20, 2019
Selective Single Molecule Nanopore Sensing of microRNA Using PNA Functionalized Magnetic Core-Shell Fe3O4-Au
Hao Wang1, Haoran Tang1, Cheng Yang1
1Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science , Anhui Normal University , Wuhu 241000 , P. R. China.
Abstract:
Solid-state nanopores have been employed as useful tools for single molecule analysis due to their advantages of easy fabrication and controllable diameter, but selectivity is always a big concern for complicated samples. In this work, functionalized magnetic core-shell Fe3O4-Au nanoparticles, which acted as a molecular carrier, were introduced into nanopore electrochemical system for microRNA sensing in complicated samples with high sensitivity, selectivity and signal-to-noise ratio (SNR). This strategy is based on the specific affinity between neutral peptide nucleic acids (PNA)-modified Fe3O4-Au nanoparticles and negative miRNA, and the formation of negative Fe3O4-Au-PNA-miRNA complex, which can pass through the nanopore by application of a positive potential and eliminate neutral Fe3O4-Au-PNA complex. To detect miRNA in complicated samples, a magnet has been used to separate Fe3O4-Au-PNA-miRNA complex with good selectivity. We think this is a facile and effective method for the detection of different targets at single molecular level, including nucleic acids, proteins, and other small molecules, which will open up a new approach in the nanopore sensing field.
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