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Updated: Jan 14, 2026
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
Development of a Single-Particle Collision Electrochemistry Strategy for the Sensing of Matrix Metalloproteinase-9
Xiangqi Liu1, Qingshan Dai1, Yan Zhou1
1Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P. R. China.
Abstract:
As a potential biomarker associated with various diseases, high sensitivity and selective quantitative detection of matrix metalloproteinase-9 (MMP-9) are of great significance. Single-particle collision electrochemistry (SPCE) has attracted great attention in the biosensing field due to its advantages of simplicity, speed, and homogeneous detection. Herein, we developed a sensing strategy for highly sensitive and specific detection of MMP-9 using the SPCE technique. Ag nanoparticles (AgNPs) can collide on gold ultramicroelectrodes (Au UME) and produce peak collision signals due to the oxidation of AgNPs. When MMP-9 aptamers adsorb on the AgNPs surface to form AgNPs-Aptamer conjugates, the oxidation of AgNPs on Au UME is inhibited, and the collision signals are weakened. However, when target MMP-9 is present, the strong binding affinity between MMP-9 and aptamers reduces the DNA content of the AgNPs surface, thereby promoting the recovery of collision current and frequency, which can be used to detect MMP-9 with high sensitivity and selectivity. This SPCE-based strategy does not require complex biomarkers and washing procedures and can sensitively and homogeneously detect target MMP-9 molecules.
