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Updated: Jan 24, 2026

Listeria monocytogenes Infection of the Brain
Published on: October 2, 2018
[Fluorescence immunosorbent assay for quantitative detection of Listeria monocytogenes]
Weihua Huang1, Lun Li1, Chaochao Chen1
1School of Life Science, Jiangxi Normal University Nanchang 330096, China.
Objective:
Establishment of fuorescence immunosorbent assay for quantitative detection of Listeria monocytogenes.
Methods:
The coupled mAbs named 10 E7 H6 and 10 A11 were screened from seven strains of anti-L. monocytogenes mAbs using a sandwich enzyme-linked immunosorbent assay(ELISA). The fluorescent immunoassay was established for L. monocytogenes detection by using biotinylated 10 A11 mAbs as detection antibody, 10 E7 H6 mAbs as capture antibody, and streptavidin-labeled fluorescent microspheres as detection probes, respectively.
Results:
The optimum concentrations of capture and detection antibodies were 10 μg/mL and 5 μg/mL, respectively, and the optimum reaction pH was 7. 4. Under these conditions, the limit of detection of the proposed method for L. monocytogenes detection was 10~5 CFU/mL, which improved by two orders of magnitude compared to conventional ELISA; and it has a certain cross-reaction with several other Listeria but no significant cross-reactivity with other pathogenic bacteria.
Conclusion:
The method can be used for the detection of L. monocytogenes in pure culture solution, and can also be used for rapid immunological screening test of several other Listeria species in the genus Listeria.
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