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Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
Published on: June 26, 2016
Controlled isoflurane anesthesia exposure is required for reliable behavioral testing in murine surgical models
Kensuke Toyama1, Joshua M Spin2, Yasunori Abe3
1Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, United States; VA Palo Alto Health Care System, Palo Alto, CA, United States; Department of Pharmacology, Ehime University Graduate School of Medicine, Ehime, Japan.
Abstract:
We investigated the effects of variations in anesthesia exposure time prior to conducting anxiety response behavioral testing in sham controls from an experimental murine model. The staying time in the center area of the Open Field test in the "long exposure" group was significantly decreased compared to that of the "short exposure" group. Significant correlation was found between anesthesia time and the duration of staying time in the center area. We conclude that anesthesia time may have a significant impact on behavioral anxiety testing in this context, and advise careful control of this parameter in protocol optimization in related surgical animal models.
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