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Updated: Jan 24, 2026

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
Published on: October 8, 2013
Comparison of a Rapid Plate Count and MPN Methods for Enumeration of Fecal Coliforms and Escherichia coli in
Guadalupe R Garcia1, Robert E Haymond1, Diane M Sprague1
1Food and Drug Administration, 1Denver Federal Center. P.O. Box 25087. Denver, Colorado 80225.
Abstract:
A direct elevated temperature plate count method utilizing modified fecal coliform agar with rosolic acid (ETPC/mFC) was compared to 5-tube and 3-tube most probable number (MPN) procedures for its accuracy in enumerating fecal coliforms and Escherichia coli in naturally and artificially contaminated soft-shell clams ( Mya arenaria ). The results indicated that the extent of overall recovery of fecal coliforms was similar in the two methods tested. Therefore, the ETPC/mFC method may be considered as a rapid procedure for fecal coliform screening during depuration of soft-shell clams.
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