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Updated: Jan 24, 2026

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
N-Nitrosamines in Nitrite-Cured Chicken-Seal Salami
F Shahidi1, J Synowiecki1, N P Sen2
1Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland AlB 3X9 Canada.
Abstract:
The effect of inclusion of mechanically separated seal meat (MSSM) and seal protein hydrolyzate (SPH) on the formation of N-nitrosamines in chicken salami was investigated. Inclusion of MSSM (10 or 20%) or SPH (1 or 2%) in salami had little effect on the formation of N-nitrosodimethylamine (NDMA) in the products. While the control sample contained 0.33 μg/kg NDMA, other samples contained 0.37 to 0.52 μg/kg of NDMA. Smoking of the samples resulted in small but an insignificant difference in the content of NDMA in the products. Some products contained traces of N-nitrosopiperidine, N-nitrosopyrrolidine and N-nitrosomorpholine, which were perhaps formed from interaction of amines in spice mixes with nitrite. Boiling, microwaving and frying had little effect on the content of N-nitrosamines; fried products contained up to 0.79 μg/kg NDMA.
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Structural Isomerism
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...

