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Updated: Jan 24, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Development and optimization of an immunoassay for the detection of Hg(II) in lake water
Xuexue Jin1, Rong He1, Xingrong Ju1
1College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing China.
Abstract:
In this paper, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb-A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween-20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC50) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti-Hg mAbs also have some affinity with methyl mercury (CH3Hg) and with no cross-reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.
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