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Updated: Jan 24, 2026

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
Published on: March 2, 2020
Induction of Tendon-Specific Markers in Adipose-Derived Stem Cells in Serum-Free Culture Conditions
Noelia D Falcon1, Graham P Riley2, Aram Saeed1
11School of Pharmacy, University of East Anglia, Norwich, United Kingdom.
Impact Statement:
Herein, we describe the tenogenic effect of bone morphogenetic protein-12 and transforming growth factor-β1 in cultured adipose-derived stem cells (ADSCs) in serum-free conditions. This culture system provides an insight into serum-free culture conditions in stem cell differentiation protocols. A positive response of the ADSCs to the tenogenic induction was observed. In particular, the different growth factors used in this study displayed notable differences both on the gene and on the protein expression of the tendon-specific markers. The results underline the positive outcome of the serum removal in tenogenic differentiation protocols, contributing to the development of future cell-based therapies for tendon regeneration and repair.
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