Related Experiment Video
Updated: Jan 24, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
In vivo corneal confocal microscopy aided by optical coherence tomography
Sebastian Bohn1,2,3,4, Karsten Sperlich1,2,3,5, Heinrich Stolz6
1Department of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Abstract:
In vivo corneal confocal microscopy and its operability in scientific as well as in clinical applications is often impaired by the lack of information on imaging plane position and orientation inside the cornea during patient's examination. To overcome this hurdle, we have developed a novel corneal imaging system based on a commercial scanning device and a modified Rostock Cornea Module. The presented preliminary system produces en face images by confocal laser scanning microscopy and sagittal cross-section images by optical coherence tomography simultaneously. This enables imaging guidance during examinations, improved features for diagnostics along with thickness measurements of the cornea as well as corneal substructures from oblique sections.
Related Concept Videos
Confocal Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Positron Emission Tomography
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
Properties of Enantiomers and Optical Activity
Imaging Studies III: Computed Tomography

