Modification of the 6-hydroxydopamine technique for the correct determination of superoxide dismutase

Heikkila & Cabbat (Anal. Biochem. 75, 356-362 (1976] have proposed the autoxidation of the 6-hydroxydopamine as a method to test superoxide dismutase activity in biological samples. This method has several advantages but in some instances leads to incorrect determinations. We present here a necessary modification of the method to avoid bias.