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Immunonephelometric quantification of free haemoglobin
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|June 1, 1987
Summary
This study introduces a fast, automated immunonephelometric assay for measuring free hemoglobin in blood samples. The method offers high precision and accuracy, suitable for clinical diagnostics.
Area of Science:
- Clinical chemistry
- Immunology
- Hematology
Background:
- Accurate quantification of free hemoglobin is crucial for diagnosing hemolytic conditions.
- Existing methods may lack speed, specificity, or automation.
Purpose of the Study:
- To describe a fully mechanized immunonephelometric method for free hemoglobin determination.
- To evaluate the method's speed, precision, accuracy, and applicability to different sample types.
Main Methods:
- Utilized rabbit antiserum against human hemoglobin and the Behring Nephelometer Analyser.
- Employed an immunonephelometric approach for quantitative analysis.
- Tested on serum, plasma (citrate, heparin, EDTA), and aqueous hemolysates.
Main Results:
- Achieved rapid determination (6 minutes) with a small sample volume (250 microliters).
- Demonstrated a wide measuring range (9-2300 mg/ml) and good precision (CVs as low as 1.6% intra-assay, 5.6% inter-assay).
- Showed high accuracy, correlating closely with cyanomethemoglobin spectrometry (r=0.9837), though lipemic samples may interfere. EDTA plasma was unsuitable.
Conclusions:
- The described method provides a rapid, specific, and precise means for free hemoglobin measurement.
- The assay is suitable for routine clinical laboratory use, with considerations for sample type and potential interferences.