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Mangiferin improves hepatic damage-associated molecular patterns, lipid metabolic disorder and mitochondrial

Mengran Li1, Chunxiao Wu, Hongbin Guo

  • 1College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, 180 WuSi Road, Lianchi District, Baoding 071002, China. xuefanone@163.com.

Food & Function
|May 31, 2019
PubMed

Insights

Mangiferin (MF) effectively treats alcohol hepatitis (AH) in rats by regulating liver function, metabolic pathways, and key gene expressions. This natural compound shows promise in preventing AH progression through multiple molecular mechanisms.

Area of Science:

  • Hepatology
  • Pharmacology
  • Biochemistry

Background:

  • Alcohol hepatitis (AH) is a severe liver condition often linked to lipid metabolic disorders and mitochondrial dysfunction.
  • Damage-associated molecular patterns (DAMPs) play a crucial role in the pathogenesis of AH.

Purpose of the Study:

  • To investigate the therapeutic effects of mangiferin (MF) on alcohol hepatitis (AH) in a rat model.
  • To elucidate the underlying mechanisms of MF's action, focusing on DAMPs, lipid metabolism, and mitochondrial function.

Main Methods:

  • Rats with induced AH were treated with MF.
  • Serum biochemistry, liver function markers, and levels of specific metabolites were analyzed.
  • Gene and protein expression of key molecules involved in inflammation, lipid metabolism, and mitochondrial dynamics were assessed using RT-qPCR and Western blotting.

Main Results:

  • MF significantly improved liver function and normalized serum levels of alcohol, free fatty acids (FFAs), and metal elements.
  • MF modulated the expression of genes and proteins including PPAR-γ, OPA-1, Cav-1, EB1, NF-κB p65, NLRP3, Cas-1, IL-1β, and reduced markers of apoptosis (C-CAS-3, C-PARP).
  • MF altered metabolic pathways, including phenylalanine metabolism, the TCA cycle, and ascorbate and aldarate metabolism, by adjusting levels of specific metabolites.

Conclusions:

  • Mangiferin demonstrates significant protective effects against alcohol hepatitis in rats.
  • MF acts by modulating DAMPs, lipid metabolism, mitochondrial function, and inflammatory pathways.
  • MF represents a potential therapeutic agent for alcohol hepatitis, acting through diverse molecular targets and metabolic adjustments.

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