Ottowia flava sp. nov., isolated from fish intestines

Area of Science:

• Microbiology
• Bacteriology
• Genomics

Background:

• The genus Ottowia comprises Gram-negative bacteria with diverse ecological roles.
• Understanding bacterial diversity in aquatic environments is crucial for ecological and biotechnological applications.

Purpose of the Study:

• To characterize a novel bacterial strain isolated from fish intestines.
• To determine the taxonomic placement of the novel strain within the genus Ottowia.

Main Methods:

• Isolation and cultivation of bacteria from fish intestines.
• 16S rRNA gene sequencing for phylogenetic analysis.
• DNA-DNA hybridization for genetic relatedness assessment.
• Fatty acid analysis, respiratory quinone determination, and polar lipid profiling for chemotaxonomic characterization.
• Determination of optimal growth conditions (pH, temperature, salinity).

Main Results:

• A novel non-motile, short rod-shaped, Gram-negative bacterium, strain GY511^T, was isolated.
• Phylogenetic analysis based on 16S rRNA gene sequences showed highest similarity to O. oryzae NBRC 113109^T (97.6%).
• DNA-DNA hybridization values indicated distinctness from related species (35.4% with O. oryzae NBRC 113109^T).
• Chemotaxonomic analysis revealed major fatty acids as summed features 3 and 8, C_16:0, and ubiquinone-8 as the respiratory quinone.
• The strain exhibited aerobic growth utilizing thiosulfate as a co-substrate, oxidizing it to sulfate.
• The G+C content of the genomic DNA was 62.9 mol%.

Conclusions:

• Strain GY511^T represents a novel species within the genus Ottowia.
• The proposed name for this new species is Ottowia flava sp. nov.
• The type strain is GY511^T (=NBRC 113500^T =DSM 107425^T =CGMCC 1.13650^T).