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Published on: July 21, 2017
Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery
Mohsen Alipour1, Saman Hosseinkhani2,3
1Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract:
The delivery of nucleic acid to eukaryotic cells is challenging due to presence of various intra- and extracellular barriers and need to delivery carriers. However, current gene delivery carriers, including PLL, PEI, and liposome, suffer from nanocarrier associate toxicity, low efficiency and polydispersity, and non-biodegradability. Here we describe our strategy for developing safe, efficacious, and monodisperse peptide-based carrier for gene delivery. We explain the use of genetic engineering technology for integrating various functional motifs in a single peptide, with less than 100 nm size, which facilitated gene delivery into mammalian cell.
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