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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.4K
Atomic Orbitals02:44

Atomic Orbitals

43.5K
An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
43.5K
Intermolecular Forces03:13

Intermolecular Forces

70.5K
Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
70.5K
The Energies of Atomic Orbitals03:21

The Energies of Atomic Orbitals

30.0K
In an atom, the negatively charged electrons are attracted to the positively charged nucleus. In a multielectron atom, electron-electron repulsions are also observed. The attractive and repulsive forces are dependent on the distance between the particles, as well as the sign and magnitude of the charges on the individual particles. When the charges on the particles are opposite, they attract each other. If both particles have the same charge, they repel each other.
30.0K
Intermolecular vs Intramolecular Forces03:00

Intermolecular vs Intramolecular Forces

96.4K
Intermolecular forces (IMF) are electrostatic attractions arising from charge-charge interactions between molecules. The strength of the intermolecular force is influenced by the distance of separation between molecules. The forces significantly affect the interactions in solids and liquids, where the molecules are close together. In gases, IMFs become important only under high-pressure conditions (due to the proximity of gas molecules). Intermolecular forces dictate the physical properties of...
96.4K
Atomic Structure01:33

Atomic Structure

207.7K
Overview
207.7K

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Related Experiment Video

Updated: Jan 24, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

15.4K

Atomic Force Microscopy for Cell Membrane Investigation.

Mingjun Cai1, Hongda Wang2

  • 1Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, People's Republic of China.

Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) reveals detailed structures on both sides of cell membranes. This technique allows direct observation of membrane composition and protein locations.

Keywords:
Atomic force microscopy (AFM)Cell membrane structuresTopography and recognition imaging (TREC)

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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
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Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • The cell membrane is crucial for cellular functions but its detailed structure is not fully understood.
  • Understanding cell membrane structure is key to deciphering cellular processes like transport and signaling.

Purpose of the Study:

  • To present methods for preparing cell membranes from red blood and nucleated cells.
  • To visualize the substructural details of both sides of the cell membrane using high-resolution AFM.
  • To demonstrate the capability of AFM for observing membrane composition and locating membrane proteins.

Main Methods:

  • Preparation of cell membranes from red blood cells and nucleated cells.
  • High-resolution Atomic Force Microscopy (AFM) imaging.
  • Time-lapse AFM for dynamic observation of membrane structure.
  • Molecular recognition techniques for protein localization.

Main Results:

  • High-resolution AFM topographs successfully revealed substructural details on both surfaces of the cell membrane.
  • Time-lapse AFM enabled direct observation of the cell membrane's structural composition.
  • Molecular recognition combined with AFM allowed for the precise localization of membrane proteins.

Conclusions:

  • The developed methods enable detailed nanoscale visualization of cell membrane structures.
  • AFM is a powerful tool for elucidating cell membrane architecture and protein distribution.
  • This approach advances the study of cell membrane biology and function.