Related Experiment Video
Updated: Jan 24, 2026

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
Published on: November 14, 2010
Automated Image Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens
Giulia Galotto1, Jeffrey P Bibeau1, Luis Vidali2
1Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
Abstract:
This protocol describes the automated imaging and a quantitative analysis of the morphology of small plants from the moss Physcomitrella patens. This method can be used for the analysis of growth phenotypes produced by transient RNA interference or for the analysis of stable mutant plants. Furthermore, we describe how to acquire higher resolution images via the acquisition of a collection of multiple overlapping tiles from the same image. Information is presented to guide the investigator in the choice of vectors and basic conditions to perform transient RNA interference in moss. Detailed directions and examples for fluorescence image acquisition of small regenerating moss plants are provided. Instructions for stitching image tiles and for using an ImageJ-based macro for the quantitative morphological analysis of moss plants are also provided.
Related Concept Videos
Light Acquisition
Water and Mineral Acquisition
Cells Coordinate Growth and Proliferation
Population Growth
Microbial Morphologies
Meristems and Plant Growth

