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Analysis of Vascular Hydrogen Sulfide Biosynthesis
Thomas J Lechuga1, Dong-Bao Chen2
1Department of Obstetrics and Gynecology & Pathology, University of California Irvine, Irvine, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
Summary
Estrogen regulates hydrogen sulfide (H2S) production in arteries by increasing cystathionine β-synthase (CBS) expression. This finding is crucial for understanding uterine blood flow regulation and potential pregnancy complications.
Area of Science:
- Biochemistry
- Physiology
- Endocrinology
Background:
- Hydrogen sulfide (H2S) is recognized as the third gasotransmitter, possessing vasodilatory and pro-angiogenic effects.
- Endogenous H2S is primarily synthesized by cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE).
Purpose of the Study:
- To investigate the regulation of vascular H2S biosynthesis by exogenous estrogens.
- To examine the role of H2S in uterine hemodynamics.
Main Methods:
- Real-time quantitative PCR (qPCR) for mRNA analysis of CBS and CSE.
- Immunoblotting for protein level assessment of CBS and CSE.
- Immunofluorescence microscopy for protein localization and quantification.
- Methylene blue assay for H2S production measurement with selective inhibitors.
Main Results:
- Estrogen replacement therapy in ovariectomized sheep stimulated CBS, but not CSE, expression in uterine and mesenteric arteries.
- Elevated H2S biosynthesis due to CBS upregulation was observed in human uterine arteries during estrogen-dominant states (proliferative phase, pregnancy).
Conclusions:
- Estrogen plays a significant role in regulating vascular H2S biosynthesis, primarily through CBS.
- H2S is implicated in uterine hemodynamics, with potential implications for pregnancy complications.
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