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06:19
Fractionation of Lignocellulosic Biomass using the OrganoCat Process
Published on: June 5, 2021
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Screening for Oily Yeasts Able to Convert Hydrolysates from Biomass to Biofuels While Maintaining Industrial Process
Patricia J Slininger1, Bruce S Dien2, Joshua C Quarterman2
1Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL, USA. Pat.Slininger@ars.usda.gov.
Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
Summary
Researchers developed efficient methods to screen oleaginous yeasts for lipid production from lignocellulosic hydrolysates. This work optimizes yeast cultivation for biodiesel and chemical applications.
Area of Science:
- Biotechnology
- Microbiology
- Renewable Energy
Background:
- Growing demand for sustainable biofuels and chemicals.
- Need for efficient oleaginous yeast strains capable of utilizing low-cost feedstocks.
- Lignocellulosic hydrolysates present a promising, cost-effective substrate for microbial lipid production.
Purpose of the Study:
- To develop and validate high-throughput screening methods for identifying oleaginous yeast strains.
- To optimize cultivation conditions for enhanced lipid production in selected yeast strains.
- To evaluate lipid quality and sugar utilization efficiency in a microcultivation format.
Main Methods:
- Utilized a 96-well aerobic microcultivation system for strain screening and condition optimization.
- Employed a suite of high-throughput assays to measure biomass, lipid accumulation, fatty acid profiles, and sugar consumption.
- Adapted and validated a gas chromatography (GC) microtechnique for analyzing yeast lipid composition.
Main Results:
- Established a robust workflow for ranking yeast strains based on lipid conversion efficiency from hydrolysate sugars.
- Optimized cultivation parameters to maximize lipid yield and quality in selected high-performing strains.
- Validated the microtechnique for accurate and efficient lipid analysis in yeast, crucial for high-throughput screening.
Conclusions:
- The described microcultivation and assay system enables rapid and efficient discovery and optimization of oleaginous yeasts.
- This approach facilitates the development of sustainable microbial processes for producing lipids from lignocellulosic biomass.
- The validated methods support the advancement of biodiesel and biochemical production from renewable resources.
Keywords:
BioconversionBiodieselHigh-throughputLignocelluloseLipid assaysLipidsMicrocultureOleaginous yeastScreenTriacylglycerolsMore Related Videos
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