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Updated: Jan 24, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
Published on: November 15, 2021
Narrowband-autofluorescence imaging for bone analysis
Laure Fauch1,2,2, Anni Palander3,2,4, Hannah Dekker5
1SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland.
Abstract:
We present a new autofluorescence-imaging method for bone analysis. This method, based on the autofluorescence of bone, provides color images in microscopic scale. The color images are created from three monochrome images acquired with optimal excitation- and emission-wavelengths combinations. The choice of these combinations were determined from the study of two-dimensional distributions of bone-features-bispectral autofluorescence in the visible- and ultraviolet-spectral range. We demonstrate that main-bone features visualized with MG-staining method can also be visualized in the autofluorescence-color image. Furthermore, the autofluorescence-color image presents features hardly distinguished in a histological-bone section.
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