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Validated LC-MS/MS method for simultaneous quantification of seven components of Naodesheng in rat serum after oral
Lan Luo1, Jiazhen Kang1, Weijun Zhao1
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Abstract:
A simple, precise and reliable LC-MS/MS method was developed and validated for simultaneous quantification of vitexin, notoginsenoside R1, hydroxysafflor yellow A, ginsenoside Rd, puerarin, daidzein and senkyunolide I as components of Naodesheng (NDS) in rat serum. The Linearity ranges in rat serum were 0.045-4.5 μg/mL for vitexin, 0.0476-4.76 μg/mL for notoginsenoside R1, 0.0422-4.22 μg/mL for hydroxysafflor yellow A, 0.0426-4.26 μg/mL for ginsenoside Rd, 0.0436-4.36 μg/mL for puerarin, 0.026-2.6 μg/mL for daidzein, and 0.05-5 μg/mL for senkyunolide I, with the correlation coefficients greater than 0.99. The established method was validated in terms of intra- and inter-day precision and accuracy, recovery, matrix effect and stability. Furthermore, the method was successfully applied for pharmacokinetic study of these seven components in rat serum after oral administration of NDS.
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