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Toward surrogate-based conservation: Preservation of European eel (Anguilla anguilla) spermatogonial stem cells.

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Eel sperm cryopreservation: An overview.

Juan German Herranz-Jusdado1, Victor Gallego1, Marina Morini1

  • 1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.

Theriogenology
|June 4, 2019
PubMed
Summary

Cryopreservation of eel sperm is crucial for aquaculture and conservation. Methanol-based protocols show improved motility and larger volumes compared to older DMSO methods, offering better prospects for eel breeding programs.

Keywords:
AnguillaDMSOFishMethanolSpermatozoa motilityVitrification

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Area of Science:

  • Aquaculture and Conservation Biology
  • Reproductive Biology
  • Cryopreservation Science

Background:

  • Eels (Anguilliformes) are commercially valuable and some species are endangered.
  • Developing effective sperm cryopreservation is vital for eel aquaculture and conservation efforts.

Purpose of the Study:

  • To review and compare different cryopreservation protocols for Japanese eel (Anguilla japonica) and European eel (Anguilla anguilla) spermatozoa.
  • To highlight advancements in cryopreservation techniques for eel sperm.

Main Methods:

  • Overview of cryopreservation protocols using dimethyl sulfoxide (DMSO) and methanol as cryoprotectants.
  • Analysis of sperm motility, fertilization rates, and potential epigenetic effects.

Main Results:

  • Early protocols using DMSO yielded moderate motility (45% for Japanese eel, 35% for European eel) but low fertilization rates and potential epigenetic changes.
  • Methanol-based protocols demonstrate improved sperm motility and allow for cryopreservation of larger sperm volumes (up to 5 mL).

Conclusions:

  • Methanol is a superior cryoprotectant for eel sperm compared to DMSO, minimizing epigenetic alterations.
  • Advanced cryopreservation techniques are essential for successful large-scale eel aquaculture and conservation programs.