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Hordein Accumulation in Developing Barley Grains.

Gregory J Tanner1, Michelle L Colgrave2,3, Malcolm J Blundell4

  • 1School of Biosciences, University of Melbourne, Melbourne, VIC, Australia.

Frontiers in Plant Science
|June 4, 2019
PubMed
Summary
This summary is machine-generated.

Barley hordein accumulation peaks at 30 days post-anthesis, with specific peptide concentrations varying. This study identifies novel hordein isoforms, crucial for understanding barley quality and breeding.

Keywords:
accumulationdeveloping barley grainglutenhordeinsmultiple reaction monitoring mass spectrometry

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Area of Science:

  • Plant Science
  • Biochemistry
  • Proteomics

Background:

  • Hordeins are the primary storage proteins in barley, influencing nutritional value and processing quality.
  • Understanding hordein accumulation is vital for crop improvement and managing immune responses.

Purpose of the Study:

  • To investigate the temporal accumulation patterns of hordein storage proteins in developing barley grains.
  • To identify and characterize novel hordein isoforms and related proteins in barley.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) for hordein quantification.
  • Western blot and liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide analysis.
  • Comparative analysis with control proteins (serpin Z4, LTP1).

Main Results:

  • Hordeins accumulated from 6 days post-anthesis (DPA), peaking at 30 DPA, with some peptides decreasing by 37 DPA.
  • LC-MS/MS identified 39 tryptic and 29 chymotryptic hordein peptides, with most peaking at 30 DPA.
  • Two novel B-hordein isoforms and two avenin-like proteins (ALPs) were identified, not previously mapped in the barley genome.

Conclusions:

  • Hordein accumulation follows a distinct temporal pattern, with specific peptide dynamics influencing overall protein concentration.
  • The identification of novel hordein isoforms and ALPs expands our understanding of barley storage proteins.
  • This research provides insights for plant breeding aimed at enhancing barley's nutritional and processing characteristics.