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Characterization and DNA Plasmid Analysis of Ropy Pediococcus spp. Strains Isolated from Basque Country Ciders.

K Fernandez1, M Dueñas1, A Irastorza1

  • 1Departamento de Química Aplicada, Facultad de Química, Universidad del País Vasco, 20080 San Sebastián, Gipuzkoa, Spain.

Journal of Food Protection
|June 5, 2019
PubMed
Summary

Ropy Pediococcus strains from spoiled cider thrive in acidic conditions and tolerate ethanol and sulfur dioxide. Plasmid DNA analysis suggests these traits, including ropiness, are encoded on plasmids.

Keywords:
Ropy phenotypecidergel electrophoresislactobacilliplasmid DNA

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Area of Science:

  • Food Microbiology
  • Bacteriology
  • Wine and Beverage Science

Background:

  • Spoilage in fermented beverages like cider can be caused by microbial contaminants.
  • Pediococcus species are known to cause spoilage, including ropiness, in various food products.

Purpose of the Study:

  • To isolate and characterize Pediococcus strains responsible for ropiness in spoiled Basque Country ciders.
  • To investigate the environmental tolerance of these strains.
  • To determine if the ropy phenotype is plasmid-encoded.

Main Methods:

  • Isolation and characterization of ten Pediococcus strains from spoiled cider.
  • Growth studies at varying pH, temperature, ethanol, and sulfur dioxide (SO2) concentrations.
  • Plasmid DNA analysis using agarose gel electrophoresis.
  • Plasmid curing experiments using ethidium bromide and novobiocin.

Main Results:

  • All ten Pediococcus strains exhibited ropiness.
  • Strains grew well above pH 3.7 and below 37°C.
  • Tolerance observed for 10% ethanol and up to 50 mg/l total SO2 (at pH 3.8).
  • Plasmid DNA analysis revealed 6 plasmid DNA bands in a selected strain.
  • Plasmid curing experiments indicated the ropy phenotype is plasmid-encoded and linked to oleandomycin resistance.

Conclusions:

  • Pediococcus strains causing cider ropiness possess specific growth and tolerance characteristics.
  • The ropy phenotype in these Pediococcus strains is likely encoded by plasmid DNA.
  • Plasmid curing not only affected ropiness but also antibiotic resistance, suggesting a link.