Related Experiment Video
Updated: Jan 24, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
A Convenient Method for Assessing Mycotoxin Production in Cultures of Aspergilli and Penicillia †
1Agriculture and Agri-Food Canada, Winnipeg Research Center, Winnipeg, Manitoba R3T 2M9.
Abstract:
Production of aflatoxins, sterigmatocystin, citrinin, and ochratoxin A by Aspergillus and Penicillium species on agar in 50-ml flasks after 21 days was detected and confirmed by thin-layer chromatography. Toxins were extracted with 2.5 ml of acidified methylene chloride for 20 to 60 min. Mycotoxin concentrations were estimated by liquid chromatography. Addition of formic acid to the methylene chloride extraction solvent effected an increase in recovery of ochratoxin A from Czapek-yeast (CY) agar from 20% to 91%. Recoveries of added mycotoxins were generally higher from CY agar than from yeast extract-sucrose (YES) agar and ranged from 11% (citrinin from YES agar) to 91% (ochratoxin A from CY agar). The method described is convenient, rapid, and reproducible, since it samples the entire culture surface. Human exposure to volatile solvents is also minimized.
More Related Videos
08:07A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products
Published on: May 22, 2019
10:04The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
Published on: September 18, 2017
Related Concept Videos
Convenience Sampling Method
Convenience sampling is a non-random method of sample selection; this method selects individuals that are easily accessible and may result in biased data. For example, a marketing...
Clinically Relevant Drug Product Specifications: Methods of Establishment
Scalar Product (Dot Product)
The scalar product of two vectors is obtained by multiplying...
Vector Product (Cross Product)
Consider the cross product of two vectors. Imagine rotating the first vector about...
Assessment of blood pressure in brachial artery(one-step method)
Prepare for the Procedure:
Assessment of blood pressure in brachial artery(two-step method)