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Homogeneous liquid-liquid microextraction based on primary amine phase separation: A novel approach for sample
Ksenia Cherkashina1, Sofya Lebedinets1, Aleksei Pochivalov1
1Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia.
Analytica Chimica Acta
|June 5, 2019
Summary
A new method uses primary amine phase separation for biological fluid pretreatment. This technique enables efficient separation and preconcentration of analytes like meropenem in human plasma and serum.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Biological fluid analysis requires effective sample pretreatment methods.
- Existing methods may lack efficiency or require complex procedures.
Purpose of the Study:
- To develop a novel homogeneous liquid-liquid microextraction technique.
- To apply this method for the separation and preconcentration of analytes in biological samples.
Main Methods:
- Development of a primary amine phase separation procedure.
- Application of the method for High-Performance Liquid Chromatography-Ultraviolet (HPLC-UV) determination.
- Analysis of meropenem in human plasma and serum.
Main Results:
- The primary amine phase separation phenomenon was studied and applied for the first time.
- Successful separation and preconcentration of meropenem.
- Linearity was observed in the 0.1-100.0 mg/L range.
- A limit of detection of 0.03 mg/L was achieved.
Conclusions:
- Homogeneous liquid-liquid microextraction based on primary amine phase separation is a viable pretreatment method.
- The technique offers efficient separation and preconcentration for biological fluid analysis.
- This approach provides a sensitive method for determining analytes like meropenem.
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