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Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella

Satheesh Nair1, Vineet Patel2,3, Tadgh Hickey2,3

  • 1Gastrointestinal Bacteria Reference Unit, Public Health England, London, United Kingdom satheesh.nair@phe.gov.uk.

Journal of Clinical Microbiology
|June 7, 2019
PubMed

Insights

A new real-time PCR assay rapidly differentiates typhoidal from nontyphoidal Salmonella. This method, using crude DNA extracts, offers faster identification for improved clinical and public health responses.

Area of Science:

  • Microbiology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Accurate differentiation of Salmonella species is crucial for managing enteric fever and implementing public health strategies.
  • Conventional identification methods like biochemistry, serotyping, and whole-genome sequencing are time-consuming, delaying critical interventions.

Purpose of the Study:

  • To develop and validate a rapid real-time PCR assay for distinguishing typhoidal Salmonella from nontyphoidal Salmonella.
  • To enable direct analysis from crude bacterial DNA extracts for faster results.

Main Methods:

  • Development of a real-time PCR assay targeting specific Salmonella markers.
  • Evaluation of the assay's performance using crude DNA extracts from bacterial colonies.
  • Comparison with existing diagnostic methods for accuracy and speed.

Main Results:

  • The developed real-time PCR assay provides rapid and accurate differentiation of typhoidal and nontyphoidal Salmonella.
  • The assay can be performed directly on crude DNA extracts, significantly reducing turnaround time compared to traditional methods.

Conclusions:

  • This real-time PCR assay offers a faster, more efficient method for identifying Salmonella species relevant to enteric fever.
  • The assay has the potential to improve clinical decision-making, laboratory risk management, and public health surveillance for Salmonella infections.
Keywords:
Nontyphoidal salmonellaeSalmonellareal-time PCRtyphoidal salmonellaewhole-genome sequencing

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