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Updated: Jan 23, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Fast and sensitive detection of Japanese encephalitis virus based on a magnetic molecular imprinted polymer-resonance
Lianghui Luo1, Junyu Yang1, Kunsong Liang1
1Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Abstract:
A magnetic surface molecularly imprinted-resonance light scattering sensor was developed for rapid and highly sensitive detection of Japanese encephalitis virus (JEV). To prepare the surface imprinted polymer, Fe3O4 microspheres were selected as imprinting substrates which coated by silicon. Aminopropyl-triethoxysilane (APTES) as functional monomers for fixing template molecules JEV through a polymerization process of tetraethyl-orthosilicate (TEOS). The target virus JEV could be captured by the imprinted particles fastly and selectively, resulting in an increase of the RLS intensity. The results of RLS analysis proved that the obtained imprinted nanoparticles exhibited excellent specific recognition ability and high selectivity for the template virus (JEV). Furthermore, the response time of the sensor is within 20 min, which is much shorter than the previous works. The sensor with convenient separation and the limit of detection was 1.3 pM. These experimental results show that the proposed strategy is expected to achieve rapid and sensitive detection of JEV in practical applications.
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