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Updated: Jan 23, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Turn-on fluorescence detection of cysteine with glutathione protected silver nanoclusters
Nan Cao1, Huangmei Zhou1, Hao Tan1
1State Key Laboratory of Precision Spectroscopy, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, People's Republic of China.
Abstract:
In this work, we report a sensitive and selective turn-on fluorescence detection of cysteine with glutathione protected silver nanoclusters (GSH-Ag NCs). The glutathione stabilized silver nanoclusters were synthesized by the boiling water method. When excited at 380 nm, the GSH-Ag NCs exhibited a weak emission at about 680 nm, which could be enhanced by cysteine. The proposed method allows evaluation of cysteine in the range of 2-3000 μM with a detection limit of 0.51 μM. The recoveries were found to be 95.07%-101.38% when detecting cysteine contents in fetal bovine serum samples. In addition, we also discussed the possible mechanism for the fluorescence enhancement of GSH-Ag NCs by addition of cysteine. It might be the formation of cysteine and glutathione co-capped Ag NCs. This work reported a fluorimetric method for the assay of cysteine and provided a strategy for the synthesis of dual ligand-protected Ag nanoclusters.
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