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Updated: Jan 23, 2026

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody mAb by Neutralizing TNF Using an In Vitro Bioanalytical Method
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The role of TNF-α in chordoma progression and inflammatory pathways

Sukru Gulluoglu1,2, Emre Can Tuysuz1,2, Mesut Sahin3

  • 1Department of Medical Genetics, Yeditepe University Medical School, Koftuncu Sok. Acıbadem Mah. Istek Vakfi Kat: 3 34718 No: 57/1 Kadikoy, 34755, Istanbul, Turkey.

Cellular Oncology (Dordrecht, Netherlands)
|June 9, 2019
PubMed
Abstract

Insights

Tumor necrosis factor-alpha (TNF-α) promotes chordoma progression by increasing cell migration, invasion, and chemo-resistance. Elevated TNF-α correlates with poor prognosis and tumor-promoting inflammation in chordoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Chordomas are aggressive primary bone tumors known for therapy resistance and high recurrence rates.
  • These tumors can cause significant local destruction, posing a therapeutic challenge.

Purpose of the Study:

  • To investigate the impact of tumor necrosis factor-alpha (TNF-α) on chordoma progression.
  • To evaluate the relationship between TNF-α and clinical outcomes in chordoma patients.

Main Methods:

  • Chordoma cells were treated with TNF-α, and effects on chemo-resistance, epithelial to mesenchymal transition (EMT), migration, and stem cell properties were assessed.
  • Gene expression (qRT-PCR, microarray) and functional assays were employed.
  • Correlation between TNF-α expression and patient clinicopathological features was analyzed.

Main Results:

  • TNF-α enhanced chordoma cell migration, invasion, and chemo-resistance, alongside increased EMT marker expression and NF-κB activation.
  • Enhanced tumor sphere and soft agar colony formation, indicative of stem-like properties, were observed.
  • High TNF-α and TNFR1 expression correlated with tumor volume, poor survival, and elevated LIF, PD-L1, and Ki67 levels.

Conclusions:

  • TNF-α may act as a prognostic biomarker for chordoma progression.
  • Tumor-promoting inflammation, mediated by factors like TNF-α, appears crucial in chordoma advancement.
Keywords:
ChordomaInflammationTNF-αTNFRTumor size

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