Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer analysis of Feulgen hydrolysis kinetics.

C Pöppe, C Pellicciari, K Bachmann

    Histochemistry
    |February 26, 1979
    PubMed
    Summary

    This study introduces a new computational method for analyzing Feulgen hydrolysis time curves. The technique accurately fits data to the Bateman function, offering insights into its practical applications and limitations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Automatic generation of alloreactivity-reduced donor lymphocytes and hematopoietic stem cells from the same mobilized apheresis product.

    Journal of translational medicine·2023
    Same author

    Adjusting the RAPID score with 2 additional variables significantly increases its predictive value in patients with empyema.

    Scientific reports·2023
    Same author

    Basal cell carcinoma risk and solar UV exposure in occupationally relevant anatomic sites: do histological subtype, tumor localization and Fitzpatrick phototype play a role? A population-based case-control study.

    Journal of occupational medicine and toxicology (London, England)·2020
    Same author

    What predicts the outcome in patients with intestinal ischemia? A single center experience.

    Journal of visceral surgery·2019
    Same author

    Determination of reference intervals and comparison of venous blood gas parameters using a standard and nonstandard collection method in 51 dogs.

    Schweizer Archiv fur Tierheilkunde·2018
    Same author

    Is ultraviolet exposure acquired at work the most important risk factor for cutaneous squamous cell carcinoma? Results of the population-based case-control study FB-181.

    The British journal of dermatology·2017

    Area of Science:

    • Biochemistry
    • Computational Biology
    • Molecular Biology

    Background:

    • Feulgen hydrolysis is a key cytochemical staining method.
    • Analyzing hydrolysis time curves is crucial for accurate DNA quantification.
    • Existing methods for fitting these curves can be computationally intensive.

    Purpose of the Study:

    • To develop and evaluate an efficient computational method for fitting Feulgen hydrolysis time curves.
    • To apply a least squares fit to the Bateman function using adapted parameter transformation.
    • To assess the utility and constraints of the developed computer evaluation method.

    Main Methods:

    • Least squares fitting of Feulgen hydrolysis time curves.
    • Application of the Bateman function for curve analysis.
    • Utilized an adapted parameter transformation and conjugate gradients iteration procedure.
    • Tested the method on a large dataset of measured experimental data.

    Main Results:

    • Successfully fitted experimental Feulgen hydrolysis time curves to the Bateman function.
    • Demonstrated the effectiveness of the adapted parameter transformation and iteration procedure.
    • The computational method proved robust across a large number of data sets.

    Conclusions:

    • The developed computational method provides an effective approach for analyzing Feulgen hydrolysis kinetics.
    • The study discusses the practical use and inherent limitations of computer-based evaluation for this technique.
    • This method enhances the accuracy and efficiency of DNA content analysis in cytochemistry.

    Related Experiment Videos